The objectives of this research was 1) to measure the development of mouth opening width and 2) to analyze the digestive enzyme activities. The research was conducted from April to November 2017 in the Hatchery Laboratory, Faculty of Fisheries and Marine Sciences, University of Riau. The results showed that the increase of mouth opening and the rate of gut evacuation of sheatfish larvae was due to the increase of digestive enzyme activities and feed consumption rate.